Anti-oxidant 1 (ATX1) expression under Cd stress in domestic brassica oleracea varieties and their reactome analysis
BSc student: Zerina Duhović
Mentor: Assoc. Prof. Dr. Ragab Abdel-Gawad
Cadmium Chloride (CdCl2), a toxic heavy metal, is an important pollutant in the environment, toxic to most organisms and a potential threat to human health. Brassica oleracea is a member of the cabbage family Brassicaceae, which has many beneficial characteristics for our health. This study is carried out to investigate the expression of the Anti-Oxidant 1(ATX1) gene in domestic wild cabbage (Brassica oleracea), under Cadmium Chloride stress and to support the obtained data with reactome analysis. The expression of Anti- oxidant (ATX1) gene is examined on wild cabbage which is an annual plant, grown from seeds with natural resistance to diseases and changes in temperature and also with the ability to tolerate and accumulate toxic materials such as Cadmium Chloride. This plant is very beneficial for our health; it reduces the risk of several types of cancer and degenerative diseases. Cadmium due to its toxicity alters various biochemical processes in plants, such as disturbance of photosynthesis and transpiration, uptake of minerals, protein synthesis and it also causes oxidative stress. Based on previous researches, the ATX1 is protein-coding gene that controls root growth by regulating cell cycle duration, cell production, and the transition from cell proliferation in the root apical meristem (RAM) to cell elongation. After extent research, the results we obtained show that there is a decrease in the gene expression level of ATX 1 with the increasing amounts of CdCl2 supplied, as compared with the control sample. This result suggests that CdCl2 inhibits the expression of the ATX1 gene at higher Cd levels.
