Characterization of solvents and optimization of stability of compounds used in cell culture treatments
BSc student: Ada Adilovic
Mentor: Assist. Prof. Dr. Mirza Suljagic
Phytochemicals represent one of the rising agents in cancer research today. Since they can be found in many different sources, namely plants, they are characterized as one of the most numerous chemicals today. Their effects in vitro and in vivo have been tested frequently as seen through many studies. However, the bioavailability of phytochemicals still represents the major issue in in vitro and in vivo research. This report focuses on three phytochemicals including Thymoquinone (TQ), Curcumin and Quercetin in a diffuse large B cell lymphoma (DLBCL) model. In order to fully demonstrate the impact of phytochemicals on the DLBCL model, three different, but highly widespread solvents were used including DSMO, PBS and Ethanol. The determination of a compound and solvent association is of high importance when improving the stability of a compound which in return, provides more information on the efficiency of compound/solvent relation. In order to conclude how stability and solubility of substances affect cancer cell metabolism, we designed experiments that included the application of different variations of the solvent and phytochemical concentrations. The results showed uttermost consistency with decreased cell viability in a dose-dependent manner. In addition, we demonstrated that the choice of the solvent is of great significance to the phytochemical attainment.
